Effect of MUC5AC suppression on cell invasion. (A) Cell invasion through membrane filter coated with Matrigel was examined. 72 h later, invading cancer cells were stained by hematoxylin and counted under a microscope. A phase contrast photograph of SW-1990 shows the representative adhering cells to the well coated in finbonectin (arrows). Scale bar, 50 μm. (B) The number of invading si-SW1990 and si-BxPC3 was significantly lower compared to parental cells. Data shown are means ± SD. ***; P < 0.001.