mRNA and protein expression profiles of cancer cell lines. (A) mRNA expression profile of cell lines. mRNA associated with cell adhesion and invasion was determined by RT-PCR. α3, α9 and β3 integrin, MMP-3 and VEGF mRNA expression (white arrows) of si-SW1990 was down-regulated, as compared to SW1990. MMP-3, VEGF, and α3-integrin were decreased in si-BxPC3. PCI-64 which expressed no MUC5AC did not reveal MMP-3, α3-integrin mRNA expression. (B) Western blotting. Reduced MMP-3 and alpha3-integrin expression in si-SW1990 and si-BxPC3 were verified by western blotting. Phosphorylation of VEGFR-1 and phosphorylation of Erk1/2 were decreased in both of si-SW1990 and si-BxPC3 compared with parental cells (white arrow). (C) ELISA. Cell culture supernatants were examined for production of VEGF-A. VEGF-A production by si-SW1990 and si-BxPC3 was significantly decreased than parental cells. Data are means ± SD. ***; P < 0.001.