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Figure 1 | Journal of Experimental & Clinical Cancer Research

Figure 1

From: Efficient inhibition of lung cancer in murine model by plasmid-encoding VEGF short hairpin RNA in combination with low-dose DDP

Figure 1

Attenuation of VEGF expression in vitro. A) Photograph of agarose gel. Cultured A549 cells were transfected with pshVEGF or pshHK. Forty-eight hours after transfection, VEGF mRNA was semiquantified by RT-PCR. The β-actin gene was used as the internal control. B) Densitometric analysis of VEGF mRNA was done and the relative expression of each band was normalized to the blank control (5% GS). C) Forty-eight hours after transfection, supernatants were collected and assayed for secreted VEGF protein by ELISA. The results are presented as mean ± SD (n = 3). P < 0.05 versus pshHK.

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