Figure 2
From: Downregulation of SPARC expression decreases gastric cancer cellular invasion and survival
Effect of SPARC knockdown on cell migration in gastric cancer cell lines MGC 803 and HGC 27 cells. A. SPARC expression in MGC 803 and HGC 27, control and SPARC siRNA transfected cells was detected by immunoblot analysis using a rabbit polyclonal SPARC antibody (1:500). β-actin was used as loading control. B. Effects of SPARC knockdown on cell migration in gastric cancer cell lines. SPARC expression was knocked down in MGC 803 and HGC 27 cells using SPARC siRNA and subjected to a migration assay using a two-chambered invasion apparatus as described in Materials and Methods, histogram showing percent inhibition of MGC 803 and HGC 27 cell invasion. The experiment was done in triplicate and the value obtained from scrambled siRNA transfected cells was set as 100%.