Figure 4

SPARC knockdown results in induction of apoptosis in gastric cancer cell lines. For flow cytometric analysis, cells were harvested 96 h after transfection with SPARC siRNA or negative control siRNA, then stained with annexin V-FITC and propidium iodide (PI). the left half data represent data obtained from MGC 803 cells and the right ones represent data obtained from HGC 27 cells. The percentages of annexin V/PI(early apoptotic) and annexin V/PI(late apoptotic) cells is shown in each panel. Values in bold indicate decreasing SPARC expression increased apoptosis by 65% in MGC803 and 92% in HGC27 compared with negative control siRNA.