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Table 1 Primer sequences and reaction conditions for genotyping IL-10 polymorphisms

From: Association of interleukin-10 gene polymorphisms with breast cancer in a Chinese population

Polymorphism db SNP ID PCR Primer sequence RE Product size(bp)
-1082 A/G Rs1800870 F: 5'-CTCGCTGCAACCCAACTGGC-3'
R: 5'-TCTTACCTATCCCTACTTCC-3'
MnlI G: 106+33
A: 139
-819 T/C Rs1800871 F: 5-TCATTCTATGTGCTGGAGATGG-3'
R: 5'-TGGGGGAAGTGGGTAAGAGT-3'
MaeIII C:125+84
T: 209
-592 A/C Rs1800872 F: 5-GGTGAGCACTACCTGACTAGC-3'
R: 5'-CCTAGGTCACAGTGACGTGG-3'
RsaI A:236+176
C: 412
  1. Abbreviations: dbSNP ID, database identifier; SNP, single-nucleotide polymorphism; PCR, polymerase chain reaction; RE, restriction endonuclease.