Figure 2

Identification of stable A549 cell clones for RNA and protein expression.a. RT-PCR detection of RNA expression of p16INK4a (lane 1), p14ARF (lane 2) and p12 (lane 3). The products were analyzed by 1% agarose gel electrophoresis. Lane M was loaded with DL 2000 DNA marker, with sizes shown on the left. b. Immunocytochemical assays detected expression of p16INK4a, p14ARF and p12 proteins in the cell clones. No specific signal was detected in the negative control (pcDNA3 vector-transfected cells).