Figure 5
Cell growth inhibition and cell cycle redistribution effects of p16INK4a in A549 cells. Cell growth curve in a representative experiment (a) and analysis of the differential inhibition effects of the three variants in three independent experiments carried out after 72 h of subculture (b). Significant growth retardance was exerted by p16INK4a compared with the control. The protein was transduced the day before cell counting. Data shown are the mean ± standard deviation of triplicate wells or experiments. **p < 0.01. c. p16INK4a protein caused evident accumulation of A549 cells in G1 phase and a decrease of those in S phase at 48 h after subculture. Data shown are the mean ± standard deviation of three independent experiments. *p < 0.05.