Figure 4

The SDF-1 protein expression in cocultured medium of mammosphere cells with CAFs and NFs. The SDF-1 protein level in the medium conditioned by the CAFs was (426.4 ± 30.6) (pg/ml) (middle), compared to the levels produced by mammosphere cells alone (283.6 ± 35.1) (pg/ml) (left), P < 0.05. The cocultured medium of mammosphere cells with NFs (right) showed a far lower level of SDF-1(52.9. ± 13.1) (pg/ml) secretion when compared with mammosphere cells alone, P < 0.01. The SDF-1 level was measured three times in each experiment.