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Table 1 ROI values of Female Luciferase reporter mice*

From: Thymoquinone and cisplatin as a therapeutic combination in lung cancer: In vitro and in vivo

  Control TQ5 mg/kg TQ20 mg/kg
Pre-Screen 15,490 +/- 2,108 17,155 +/- 8,957 11,990 +/- 3,031
LPS 3 hrs 176,375 +/- 63,901 89,457 +/- 24,084 75,923 +/- 33,793
LPS 24 hrs 23,978 +/- 5,501 24,177 +/- 6,830 39,823 +/- 13,631
  1. NF-κB expression was measured by quantitating the luminescence intensity in regions of interest (ROI) using Living Image® 3.0 software (Caliper Life Sciences, Inc. Hopkinton, MA). (*) ROI values include +/- standard error (n = 3-4) obtained using Living Image Software version 3.0. ROI values are equal in the mice pre-treated with vehicle or TQ showing TQ has no effect on NF-κB expression. 3 hrs after LPS injection ROI values representing NF-κB expression are much lower in mice pre-treated with TQ at 5 and 20 mg/kg though not statistically significant (P < 0.10) as compared to control suggesting pre-treatment with TQ suppresses NF-κB expression. ROI return to baseline at 24 hrs in both groups.