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Figure 3 | Journal of Experimental & Clinical Cancer Research

Figure 3

From: The E-cadherin repressor slug and progression of human extrahepatic hilar cholangiocarcinoma

Figure 3

A Expression of E-cadherin in QBC939 cells. The reaction product of in vitro ribozyme cleavage was analyzed by absolute real-time quantitative PCR. The amplification plots and standard curve were obtained with the in vitro transcript from E-Cadherin. Serial 10-fold dilutions with 9 × 108 to 9 × 10-2 pg per reaction well were made in EASY Dilution (Takara). Amplification was repeated three times for each dilution. It showed Slug inhibition increased E-cadherin expression in QBC939 cells. B Evaluation of transfection efficiencies. It showed the transfection efficiency was 31.4% 48 h after siRNA-Slug transfection.

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