Figure 5

Adaphostin (ADA) induction of HMOX1 and NQO1 is inhibited by the presence of wortmannin (WTM). NCI-H522 cells were pretreated 30 minutes with 500 nM WTM, followed by incubation with 1 μM ADA. Expression of HMOX1, NQO1 and GCLC was measured by quantitative real-time reverse transcription-PCR after a further 1, 4 and 6 h and expressed as a percentage of the control ADA-induced gene expression measured at that time in the absence of WTM pretreatment. There was a significant decrease in 6 h ADA-induced HMOX1 and NQO1expression after wortmannin pretreatment (n = 3; +/- SD; ** indicates p < 0.01)