Figure 2

Transfection and silencing of TLR4 expression using three different siRNAs in human breast cancer cell line MDA-MB-231. A, RT-PCR of TLR4 from pGenesil-1 vector, ScrambledsiRNA, TLR4AsiRNA, TLR4BsiRNA and TLR4CsiRNA transfected MDA-MB-231. B, the decreased expression of TLR4 at mRNA level in pGenesil-1 vector, ScrambledsiRNA, TLR4AsiRNA, TLR4BsiRNA and TLR4CsiRNA transfected MDA-MB-231 with real-time PCR. C, analysis of transfected cells for TLR4 expression by flow cytometry. All results are representative of three separate experiments.