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Table 1 Expression of TK gene detected with real-time PCR

From: Targeted gene therapy of nasopharyngeal cancer in vitro and in vivo by enhanced thymidine kinase expression driven by human TERT promoter and CMV enhancer

Sample

Copy number

(β-actin)

Copy number

(TK)

Relative folds to β-actin

1

6.67E+07

2.78E+08

4.16792*

2

4.50E+07

1.13E+08

2.51111**

3

7.76E+07

2.17E+05

0.00279639

4

8.21E+07

Undetermined

Undetermined

5

1.69E+08

1.39E+08

0.822485

  1. Numbers 1, 2, 3, 4, 5 correspond to the numbers in Figure 3.
  2. 1: NPC 5-8F cells transfected with pGL3-basic- hTERTp-TK- EGFP- CMV;
  3. 2: MCF-7 transfected with pGL3-basic- hTERTp-TK- EGFP- CMV;
  4. 3: NPC 5-8F cells transfected with pGL3-basic- hTERTp-TK- EGFP;
  5. 4: NPC 5-8F cells transfected with pGL3-basic -TK-EGFP;
  6. 5: ECV cells transfected with pGL3-basic- hTERTp-TK- EGFP- CMV.
  7. Data are presented as mean ± standard deviation from these experiments. *P < 0.0001 for sample 1 vs sample 3, sample 1 vs sample 5 and sample 2 vs sample 3. **P < 0.001 for sample 2 vs sample 5.
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Journal of Experimental & Clinical Cancer Research

ISSN: 1756-9966

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