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Table 1 Expression of TK gene detected with real-time PCR

From: Targeted gene therapy of nasopharyngeal cancer in vitro and in vivo by enhanced thymidine kinase expression driven by human TERT promoter and CMV enhancer

Sample Copy number
(β-actin)
Copy number
(TK)
Relative folds to β-actin
1 6.67E+07 2.78E+08 4.16792*
2 4.50E+07 1.13E+08 2.51111**
3 7.76E+07 2.17E+05 0.00279639
4 8.21E+07 Undetermined Undetermined
5 1.69E+08 1.39E+08 0.822485
  1. Numbers 1, 2, 3, 4, 5 correspond to the numbers in Figure 3.
  2. 1: NPC 5-8F cells transfected with pGL3-basic- hTERTp-TK- EGFP- CMV;
  3. 2: MCF-7 transfected with pGL3-basic- hTERTp-TK- EGFP- CMV;
  4. 3: NPC 5-8F cells transfected with pGL3-basic- hTERTp-TK- EGFP;
  5. 4: NPC 5-8F cells transfected with pGL3-basic -TK-EGFP;
  6. 5: ECV cells transfected with pGL3-basic- hTERTp-TK- EGFP- CMV.
  7. Data are presented as mean ± standard deviation from these experiments. *P < 0.0001 for sample 1 vs sample 3, sample 1 vs sample 5 and sample 2 vs sample 3. **P < 0.001 for sample 2 vs sample 5.