Figure 1
From: Klotho inhibits growth and promotes apoptosis in human lung cancer cell line A549
Relative klotho gene transcripts by qRT-PCR. (A) A549 and HEK-293 cells transfected with either MYC-tagged klotho expressison vector (MYC-KL) or an entry vector (pCMV6). (B) A549 cells transfected with four klotho directed-shRNAs and a negative control-shRNA (shRNAc). Data shown are the mean results ± SD of a representative experiment performed in triplicate (n = 3), * indicates p < 0.01. Statistical comparisons showed that our klotho expression plasmid and klotho-specific shRNA could work efficiently.