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Figure 2 | Journal of Experimental & Clinical Cancer Research

Figure 2

From: Detection of DNA mismatch repair proteins in fresh human blood lymphocytes - towards a novel method for hereditary non-polyposis colorectal cancer (Lynch syndrome) screening

Figure 2

Expression of MLH1 and MSH2 proteins in fresh blood and in immortalized lymphocytes following PHA stimulation. (A) Time-dependent stimulation of MLH1 and MSH2 proteins in fresh blood lymphocytes following PHA treatment. The expression of MLH1 and MSH2 proteins increased in a time dependent manner. These proteins were often not detectable without PHA stimulation. (B) Dose response of fresh lymphocytes to PHA. Lymphocytes were stimulated with the indicated concentrations of PHA for 48 hrs. The expression of MLH1 and MSH2 proteins in fresh blood lymphocytes increased in a dose-dependent manner. (C) Dose response of immortalized lymphocytes to PHA. There was no effect of PHA on immortalized lymphocytes. MLH1 and MSH2 proteins were detectable even without PHA stimulation.

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