Figure 1From: Salivary α-amylase exhibits antiproliferative effects in primary cell cultures of rat mammary epithelial cells and human breast cancer cellsDifferences in cultures of primary mammary cells from F344 and Lewis rats and cellular localization of α-amylase. One day after preparation, epitheloids from F344 (a) showed a faster and better attachment and a more effective growth in comparison to those from Lewis rats (b). Detection of α-amylase (Cy3; red) was performed in mammary gland cells from F344 (c) and Lewis (d) rats (P1). Nuclei were stained with DAPI (blue). Pictures show cells in xy- and xz-axis by confocal microscopy. α-Amylase was present in F344 and Lewis cells. However, in Lewis cells, α-amylase was distributed throughout the whole cell, whereas in F344 cells it was found in a more granular manner near the nuclei (xz-axis).Back to article page