Wnt/β-catenin was up-regulated in tumors derived from SP cells.(A) Quantitative RT-PCR analysis revealed that the expression of β-catenin, TCF4, LEF1, CyclinD1 and c-Myc (mean ± SD) were higher in tumors derived from SP than those in tumors from non-SP. These differences were all statistically significant (* P < 0.05, ***P < 0.001). (B) Western blotting analysis showed that Wnt1, β-catenin, CyclinD1 and c-Myc in tumors derived from SP expressed higher than those in tumors from non-SP cells. The experiment was run in triplicate.