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Figure 3 | Journal of Experimental & Clinical Cancer Research

Figure 3

From: miR-15a and miR-16-1 inhibit the proliferation of leukemic cells by down-regulating WT1 protein level

Figure 3

AMO-miR-15a/16-1 reversed the expression of WT1 in K562 and HL-60 cells (A) and (B) AMO inhibited the expression of miR-15a/16-1. K562 and HL-60 cells were incubated with AMO-miR-15a/16-1 for 24 and 48 hours, then miR-15a/16-1 and U6 snRNA expression were determined by quantitative real-time PCR. (C) and (D) K562 and HL-60 cells were incubated with AMO-miR-15a/16-1 for 48 h, then mRNA and protein level of WT1 were detected by quantitative real-time PCR and Western blotting individually. *P < 0.01 versus SCR.

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