Figure 5

WT1 protein expression is inversely correlated with miR-15a or miR-16-1 expression in AML samples and normal controls. (A) WT1 protein levels from 2 normal controls (N1 and N2) and 6 AML samples (P1-P6) were measured by Western blotting. The numbers represent the relative expression of miR-15a and miR-16-1 in the same specimens. (B) and (C) Inverse correlation between miR-15a or miR-16-1 expression and WT1 protein level in 25 primary AML samples and 5 normal controls. A statistically significant correlation between miR-15a or miR-16-1 expression and WT1 protein level was observed by Pearson's method. WT1 verse miR-15a R = -0.73 P < 0.01; WT1 verse miR-16-1 R = -0.76 P < 0.01