Skip to main content
Figure 1 | Journal of Experimental & Clinical Cancer Research

Figure 1

From: MEIS1, PREP1, and PBX4 Are Differentially Expressed in Acute Lymphoblastic Leukemia: Association of MEIS1 Expression with Higher Proliferation and Chemotherapy Resistance

Figure 1

Analysis of primers used for amplification of Three-amino-acid loop-extension (TALE) family member genes. A) A pull of cDNA obtained from leukemia-derived cell lines was utilized to test the specificity and efficiency of each set of primers in the amplification of TALE family genes. After 40 cycles of amplification by conventional PCR, the PCR products were separated into 2% agarose gels and visualized under Ultraviolet (UV) light. Amplification products of the reference genes employed (RPL32 and ACTB) are also included. The 1 Kb Plus DNA Ladder (Invitrogen, Life Science) is shown in the left line; B) Amplification of PBX1 in leukemia-derived cell lines and in healthy controls separated into 2% agarose gels and visualized under UV light (upper panel). Genome map of complete (a) and alternative (b) splicing of PBX1; C) Sequence of alternative splicing of PBX1 showing adjacent coding regions of the deleted exon.

Back to article page