Figure 2
S-CLU is up-regulated in the chemo-resistant cells: A. Western blotting analysis of CLU in a panel of ovarian cancer cells. Equal amount of proteins were loaded, resolved by SDS-PAGE and immunoprobed with anti-CLU mAb. S-CLU was found in the cells and media. Some ovarian cancer cells express relatively high levels of CLU in comparison to immortalized non tumorigenic ovarian epithelial OSE cells. B. Chemo-resistant KF-TX cells shows higher expression levels of CLU compared to parental KF cells. A similar result is found in SKOV-3 compared to SKOV-3-TX cells. C. Quantitative PCR showing the difference in CLU transcript level between the TX-sensitive and TX-resistant clones in both KF (left) and Skov-3 (right) systems.