Figure 8From: The Zfx gene is expressed in human gliomas and is important in the proliferation and apoptosis of the human malignant glioma cell line U251Knocking down Zfx in human malignant cell line U251 arrested the cell cycle. Knockdown of Zfx expression induced S arrest in U251 cells. (A) Cell cycle of U251 cells was analyzed by flow cytometry. (B) S cell cycle phase determined by flow cytometry. Compared with NC, Zfx-siRNA cultures showed a significant increase in cells in S (P = 0.003; P < 0.05), compared with NC. (C) Percentage of apoptosis was plotted against U251 cell line. There was a greater amount of apoptosis in the Zfx down-regulated group of human brain glioma U251 cells (P = 0.0009, P < 0.05). The assay showed a marked induction of apoptosis with 5.51% apoptotic for NC group.Back to article page