Cloning of a novel gene, LCMR1. (A) Electrophoresis result of DDRT-PCR in 95C and 95D cells. (B) Nucleotide and amino acid sequences of LCMR1 cDNA. LCMR1 contains a 74-bp 5'- UTR, a 949-bp ORF, and a 341-bp 3'-UTR. Inframe termination (TER) codons are located at nt positions 606-608. LCMR1 encodes a 177 aa protein. (C) LCMR1 mRNA expressions in 95C and 95D cells were examined by real-time quantitative RT-PCR. LCMR1 gene expression level in 95D cells was significantly higher than in 95C cells. (*, P < 0.01) (D) LCMR1 protein expression in 95D cells was significantly higher than in 95 C cells, examined by western blot. (E) LCMR1 was differentially expressed in the various human tissue distributions by multiple tissue northern blot (MTN). Numbers indicate tissue types in columns. 1: Brain, 2: Heart, 3: Skeletal muscle, 4: Colon, 5: Thymus, 6: Spleen, 7: Kidney, 8: Liver, 9: Small intestine, 10: Placental, 11: Lung, 12: Leukocyte.