Figure 3

The effect of LS081 on ROS generation. Approximately 5 × 10⁵ K562 cells were treated for 30 min with 0.1% DMSO alone, 10 μM ferric ammonium citrate alone, 3 or 10 μM LS081 alone, or the combination of Fe and LS081 at the indicated concentrations. The cells were then incubated with DCFDA and fluorescence measured by a BD Calibur Flow cytometer expressing the fluorescence as the mean total fluorescence intensity in the gated area. Shown are the means ± SEM of 3 separate experiments with 2-3 replicates for each experiment. *** denotes P < 0.001 compared to the DMSO, Fe, or LS081 alone by 1-way ANOVA with Tukey's posttests.