Figure 1

Leukemic myelomonocitic cells express and secrete MICA and MICB. THP-1 and U937 cells (1 × 10⁷) were lysed, proteins were immunoprecipitated and equal amounts of proteins from the total lysates were resolved by SDS-PAGE and transferred to nitrocellulose membranes. The blot was developed using either anti-MICA monoclonal antibodies or anti-MICB monoclonal antibodies (A) and an appropriate secondary antibody conjugated to HRP for chemiluminescent detection. THP-1 and U937 cells (50 × 10³) were cultured in 48-well plates for 7 days, and the conditioned media were collected daily. MIC proteins were detected by ELISA assay using specific antibodies. The production of MICA and MICB was evaluated using monoclonal antibodies against MICA and MICB in THP-1 and U-937 cells (B). Standard deviations were less than 5%