Figure 4

Cervical cancer cell lines express MICA, MICB and NKG2D. CALO and INBL cells (1 × 10⁷) were lysed proteins immunoprecipitated and equal amounts of protein from total lysates were resolved by SDS-PAGE and transferred to nitrocellulose membranes. The blots were developed with either polyclonal anti-MIC antibodies (A) or monoclonal anti-NKG2D antibodies (B) and an appropriate secondary antibody conjugated to HRP for chemiluminescence detection. Flow cytometric analysis of NKG2D expression in cervical carcinoma cell lines after 72 h induction with 10 ng MICB (C). We used only MICB to induce the expression of NKG2D because we previously obtained that MICB was a better inducer of myelomonocytic cell proliferation than MICA. Graphs show NKG2D levels (solid line) and isotype controls (dotted line).