Figure 3

Effect of silibinin on Bax expression. Cells were exposed to 30 μM silibinin for various times and Bax expression was estimated by Western blot analysis. Representative (A) and quantitative (B) results of four independent experiments. (C) Cells were exposed to 30 μM silibinin for 24 h in the presence or absence of 0.5 μM calpain inhibitor (CHO) and Bax expression was estimated by Western blot analysis.