Figure 4From: An imbalance in progenitor cell populations reflects tumour progression in breast cancer primary culture modelsIsolation of putative progenitor cells from primary cultures and cell lines. A. Breast primary cultures were sorted into CALLA single-positive, EPCAM single-positive, double-positive (DP) or double-negative (DN) populations, and expressed as a percentage of total cells. B. TEM analysis revealed a high content of lipofuscin bodies in the DN population sorted from a tumour culture (arrows). C. The DN:DP ratio increased in three types of aggressive tumour (high grade, ER-negative or HER2-positive) relative to non-tumour or non-aggressive tumour cultures. D. The DN:DP ratio in metastatic MDA-MB-231 cells exceeded that in non-tumourogenic MCF-10A cells. E. Activity of the stem cell marker ALDH was similar in non-tumour versus pooled tumour cultures (left), but significantly higher in non-tumour and low grade tumour cultures compared to high grade tumour cultures (p < 0.001; right).Back to article page