Skip to main content
Figure 3 | Journal of Experimental & Clinical Cancer Research

Figure 3

From: A pilot histomorphology and hemodynamic of vasculogenic mimicry in gallbladder carcinomas in vivo and in vitro

Figure 3

Characteristic appearance and the histomorphologic observation of GBC-SD and SGC-996 xenografts in vivo. (A) GBC-SD (a 1 ) and SGC-996 (b 1 ) xenografts. Furthermore, SGC-996 xenografts exhibited different degree of tumor necrosis (red arrowhead). Immunohistochemistry with CD31 (original magnification × 200) revealed hypervascularity with a lining of ECs (red arrowheads), GBC-SD xenografts showed more angiogenesis in marginal area of tumor (a 2 ) than that of SGC-996 xenografts (b 2 ) [P = 0.0115, (B)]. Using H&E (a 3 , b 3 ) and CD31-PAS double stain (a 4 , b 4 , original magnification × 200), sections of GBC-SD xenografts showed tumor cell-lined channels containing red blood cells (a 3 , yellow circle) without any evidence of tumor necrosis. PAS-positive substances line the channel-like structures; Tumor cells form vessel-like structure with single red blood cell inside (a 4 , yellow arrowhead). However, similar phenomenon failed to occur in SGC-996 xenografts (b 3 , b 4 ) with tumor necrosis (b 3 , yellow arrowhead). TEM (original magnification × 8000) clearly visualized several red blood cells in the central of tumor nests in GBC-SD xenografts (a 5 ). Moreover, SGC-996 xenografts exhibited central tumor necrosis (b 5 , red arrowheads) which consistent with morphology changes with H&E staining.

Back to article page