Table 1 the sequences of primers.
Target gene | Primers | Annealing temperature (°C) |
|---|---|---|
Fas | Forward:5'-AGCTTGGTCTAGAGTGAAAA-3' Reverse: 5'-GAGGCAGAATCATGAGATAT-3' | 51 |
FasL | Forward: 5'-CACTTTGGGATTCTTTCCAT-3' Reverse: 5'-GTGAGTTGAGGAGCTACAGA-3' | 57 |
GAPDH | Forward: 5'-GAAGGTGAAGGTCGGATGC-3' Reverse: 5'-GAAGATGGTGATGGGATTTC-3' | 61 |