Figure 2

The expression of CCR7 mRNA and protein in Jurkat and Hut cells after CCL21 co-culture in vitro. RT-PCR amplication and Western Blot analysis of the two cell lines under the different concentration of CCL21, which was performed as described in Methods. β-actin is positive control in RT-PCR amplication and GAPDH is positive control in Western Blot analysis. The relative grey scale of CCR7 mRNA and protein in Hut cell were both higher than that in Jurkat cell with corresponding concentration of CCL21. In the group with different concentration of CCL21 of each cell lines, there were some differences on the grey scale as described in the result.