Figure 1

Analysis of exon 19 deletions by pyrosequencing. The analysis was performed with PBL DNA (A) as wild-type control and with NCI-H1650 DNA (B) as deletion control. The deletion was quantified by determining the ratio between the A₈ and A₆ peak areas. (C) The sensitivity was characterized by measuring A8/A6 ratio in different mixtures of NCI-H1650 DNA and PBL DNA.