Figure 1From: Pyrosequencing, a method approved to detect the two major EGFR mutations for anti EGFR therapy in NSCLCAnalysis of exon 19 deletions by pyrosequencing. The analysis was performed with PBL DNA (A) as wild-type control and with NCI-H1650 DNA (B) as deletion control. The deletion was quantified by determining the ratio between the A8 and A6 peak areas. (C) The sensitivity was characterized by measuring A8/A6 ratio in different mixtures of NCI-H1650 DNA and PBL DNA.Back to article page