Table 1 Sequences of primers used for pyrosequencing analysis
Exon 19 | Exon 21 | |||
|---|---|---|---|---|
primer sequence | T° of hybridation | primer sequence | T° of hybridation | |
First | 5'-GCAATATCAGCCTTAGGTGCGGCTC-3' | 58°C | 5'-CTAACGTTCGCCAGCCATAAGTCC-3' | 58°C |
PCR | 5'-CATAGAAAGTGAACATTTAGGATGTG-3' | 5'-GCTGCGAGCTCACCCAGAATGTCTGG-3' | ||
second | 5'-CATGTGGCACCATCTCACAAT-3' | 62°C | 5'-GAATTCGGATGCAGAGCTTCTT-3' | 61°C |
PCR | 5'-Biotin-CCCACA CAGCAA AGCAGAAACT-3' | 5'-Biotin-CTTTCTCTTCCGCACCCA | ||
primer for sequence reaction | 5'-TAAAATTCCCGTCGC-3' | 5'-CATGTCAAGACTACAGATT-3' |