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Table 1 Sequences of primers used for pyrosequencing analysis

From: Pyrosequencing, a method approved to detect the two major EGFR mutations for anti EGFR therapy in NSCLC

  Exon 19   Exon 21  
  primer sequence T° of hybridation primer sequence T° of hybridation
First 5'-GCAATATCAGCCTTAGGTGCGGCTC-3' 58°C 5'-CTAACGTTCGCCAGCCATAAGTCC-3' 58°C
PCR 5'-CATAGAAAGTGAACATTTAGGATGTG-3'   5'-GCTGCGAGCTCACCCAGAATGTCTGG-3'  
second 5'-CATGTGGCACCATCTCACAAT-3' 62°C 5'-GAATTCGGATGCAGAGCTTCTT-3' 61°C
PCR 5'-Biotin-CCCACA CAGCAA AGCAGAAACT-3'   5'-Biotin-CTTTCTCTTCCGCACCCA  
primer for sequence reaction 5'-TAAAATTCCCGTCGC-3'   5'-CATGTCAAGACTACAGATT-3'