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Table 2 Sequencing of wild type and mutated alleles with a particular program of pyrosquencing

From: Pyrosequencing, a method approved to detect the two major EGFR mutations for anti EGFR therapy in NSCLC

nucleotide dispensation during pyrosequencing

G

T

A

T

C

A

G

A

C

A

T

G

A

C

 

WT

 

T

A

T

C

AA

GG

AA

  

TT

 

AA

 

allelic

c.2235-2249del

 

T

A

T

C

AA AA

  

C

A

T

  

C

sequence of

c.2236-2250del

 

T

A

T

C

AA

G

A

C

A

T

  

C

 

c.2237-2251del

 

T

A

T

C

AA

GG

 

C

A

T

  

C

 

c.2240-2257del

 

T

A

T

C

AA

GG

AA

  

T

  

C

  1. Bold letters correspond to the nucleotides identical in wild type and mutated alleles; italic letters correspond to the nucleotides specific of mutated alleles.
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Journal of Experimental & Clinical Cancer Research

ISSN: 1756-9966

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