Skip to main content

Table 2 Sequencing of wild type and mutated alleles with a particular program of pyrosquencing

From: Pyrosequencing, a method approved to detect the two major EGFR mutations for anti EGFR therapy in NSCLC

nucleotide dispensation during pyrosequencing G T A T C A G A C A T G A C
  WT   T A T C AA GG AA    TT   AA  
allelic c.2235-2249del   T A T C AA AA    C A T    C
sequence of c.2236-2250del   T A T C AA G A C A T    C
  c.2237-2251del   T A T C AA GG   C A T    C
  c.2240-2257del   T A T C AA GG AA    T    C
  1. Bold letters correspond to the nucleotides identical in wild type and mutated alleles; italic letters correspond to the nucleotides specific of mutated alleles.