Cell viability (MTT assay) for determination of EC
of COX-2 stimulation in non-small cell lung cancer cell lines. (A) Prominent increasing in population of A549, H460, and A431 cells were showed in COX-2 concentration of 0, 3.82 × 10-13mol/ml, and 2.29 × 10-12mol/ml, respectively (×200). (B) Curves of cell viability (MTT assay) for determination of EC50 in A549 (y = 0.0511× + 0.0424), H460 (y = 0.0408× + 0.043), and A431 cells (y = 0.0543× + 0.0415) were showed. Calculated EC50 were 8.95 nmol/L in A549, 11.2 nmol/L in H460, and 8.44 nmol/L in A431 cells.