Figure 3

The change of HIF-1α proteins in HepG2 cells under hypoxic condition by Western blotting measurement. (A) The representative gel picture was taken from three separate experiments. (B) Compared with hypoxic control, the expression of HIF-1α was reduced in BSO concentration-dependent manner, and the analysis of relative densities showed that there was statistical difference the experimental cells by 100 and 200 μM BSO pretreatment respectively (^◆p < 0.05, ^#p < 0.01). After NAC incubation, the expression of HIF-1α was elevated again, and there were significant difference between the group with 100 μM NAC treatment and that without NAC treatment (^▲P < 0.01).