Figure 4

The change of HIF-1α expression by ICC assay. (A) The picture of ICC was shown. a: negative control; b: normoxic control; c: hypoxic control; d: the hypoxic cells by 50 μM BSO pretreatment; e: the hypoxic cells by 100 μM BSO pretreatment; f: the hypoxic cells by 200 μM BSO pretreatment; g: the hypoxic cells by 50 μM BSO + 5 mM NAC pretreatment; j: the hypoxic cells by 100 μM BSO + 5 mM NAC pretreatment; k: the hypoxic cells by 200 μM BSO + 5 mM NAC pretreatment. (B) The results of statistical analysis were shown with H-score values of semi-quantitative evaluations. (^◆P <0.05, ^#p < 0.01, compared with hypoxic control; *P <0.05, compared with the hypoxic cells by 5 mM NAC pretreatment).