RB was identified as target genes of miR-106b. (A) A schematic representation showing the putative target site for miR-106b in RB mRNA 3'UTR. (B) Cells were transfected with As-miR-106b and miR-106b, and the expression of RB was analyzed by Western blot. The expression of β-actin was used as a loading control. (C) Luciferase constructs were transfected into cells transduced with As-miR-106b and miR-106b. Luciferase activity was determined 48 h after transfection. The ratio of normalized sensor to control luciferase activity is shown. Data are expressed as the mean ± SD of 3 independent experiments. * P < 0.05 compared with control group.