Figure 3

Overexpression of Nm23 suppressed cell invasion. The invasion assay was used to determine the invasive ability of T47D cells treated with 0.5% v/v ethanol and overexpressing Nm23, independently and in combination. (A) Alcohol treatment increased the invasiveness of the T47D cells transfected with the empty vector; however, alcohol did not increase invasion in the T47D cells transfected with Nm23. (B) Western blot shows Nm23 expression levels following ethanol treatment, Nm23 overexpression, and the combination of ethanol and Nm23 overexpression. Quantification by ImageJ software indicates relative Nm23 expression. (*p < 0.05, as compared to the control cells transfected with empty vector).