Table 1 PCR reaction conditions and primer sequences

Human

VEGF-A

sense 5'-TGGAAGAAGCAGCCCATGAC-3'

59

375

antisense 5'-GCACTAGAGACAAAGACGTG-3'

IL-6

sense 5'-TCAATGAGGAGACTTGCCTG-3'

55

410

antisense 5'-GATGAGTTGTCATGTCCTGC-3'

PDGFC

sense 5'-GCCTCTTCGGGCTTCTCC-3'

56

395

antisense5'-TTACTACTCAGGTTGGATTCCGC-3'

FN1

sense 5'-CGAAATCACAGCCAGTAG-3'

51

278

antisense 5'-ATCACATCCACACGGTAG-3'

MMP28

sense 5'-CAAGCCAGTGTGGGGTCT-3'

56

252

antisense 5'-TAGCGGTCATCTCGGAAG-3'

MMP14

sense 5'-ATGTCTCCCGCCCCA-3'

60

678

antisense 5'-TCAGACCTTGTCCAGCAGG-3'

GLUT1

sense 5'-CGGGCCAAGAGTGTGCTAAA-3'

62

283

antisense 5'-TGACGATACCGGAGCCAATG-3'

GLUT2

sense 5'-CCTGAATGCCAAGGGAATCCGG-3'

48

368

antisense 5'-GCCAGATGAGGTAATCAATCATAG-3'

GAPDH

sense 5'-AGAAGGCTGGGGCTCATTTG-3'

57

258

antisense 5'-AGGGGCCATCCACAGTCTTC-3'

Chicken

VEGF-A

sense 5'-GTCTACGAACGCAGCTTCTG-3'

62

265

antisense 5'-TCACATGTCCAAGTGCGCAC-3'

IL-6

sense 5'- TTGATGGACTCCCTAAGGC-3'

50

395

antisense 5'-GATTCGGGACTGGGTTCTC-3'

PDGFC

sense 5'-TTCTCAACCTGGATTCTGC-3'

52

355

antisense 5'-AATGGTGTCAGTTCGCTTC-3'

FN1

sense 5'-ACCAACATTGACCGCCCTAA-3'

56

458

antisense 5'-AATCCCGACACGACAGCAGA-3'

MMP28

sense 5'-TGACATCCGCCTGACCTT-3'

57

376

antisense 5'-GTCCTGGAAGTGAGTGAAGACC-3'

MMP14

sense 5'-CGTGTTCAAGGAGCGGTGGC-3'

61

114

antisense 5'-TAGGCGGCGTCGATGCTGT-3'

GLUT1

sense 5'-CACTGTTGTTTCGCTCTTCG-3'

42

316

antisense 5'-AATGTACTGGAAGCCCATGC-3'

GLUT2

sense 5'-AGTTTGGCTACACTGGAG-3'

60

436

antisense 5'-AGGATGGTGACCTTCTCC-3'

GAPDH

sense 5'-CTTTCCGTGTGCCAACCC-3'

65

108

antisense 5'-CATCAGCAGCAGCCTTCACTAC-3'