Figure 2

Ad-bFGF-siRNA reduces the activation of upstream molecules and the expression of downstream molecules of STAT3 in U251 cells. (A) Ad-bFGF-siRNA (MOI = 100) reduces the phosphorylation/activation of ERK1/2 and JAK2 in a time-dependent manner in U251 cells. Total ERK1/2 and JAK2 expression remains stable. Total and phosphorylated Src decreases at 48 h in a similar manner. (B) Ad-bFGF-siRNA (MOI = 100) reduces the expression of CyclinD1 and Bcl-xl at 72 h time point.