Figure 3

Ad-bFGF-siRNA reduces IL-6 secretion in U251 cells. (A) ELISA analysis showed that IL-6 secretion in the Ad-bFGF-siRNA group (MOI = 100) was lower than that in the control and Ad-GFP groups during both 24-48 h and 48-72 h periods. **: p < 0.0001. Data are presented as mean ± SD, n = 3. (B) U251 cells infected with Ad-bFGF-siRNA for 48 h were treated with serum-free DMEM in the presence or absence of recombinant IL-6 (100 ng/ml) for 24 h. Cells treated with DMSO for 72 h served as controls. The phosphorylation of STAT3 at both Tyr705 and Ser727 is elevated after stimulated with IL-6 for 24 h.