Txnip -ROS- TRX axis regulation by hyperglycemia varies among cell lines. Cells were grown chronically in RPMI 5 or 20 mM glucose (GLC). Data is represented as fold change over 5 mM baseline, with > 1 fold change indicating an increase over baseline and < 1 a decrease over baseline levels. Multiple myeloma-derived ARH77, NCIH929 and U266B1, which showed glucose response, were grouped and the mean value ± SD for the group presented above.. A. Thioredoxin-interacting protein (TXNIP) RNA levels. B. Reactive l oxygen species (ROS)-levels. C.Thioredoxin (TRX) activity. Black star represents p-value compared to 5 mM, cross indicates p- value of MC/CAR compared to grouped value.