Figure 3

Inductive effect of CHO cells with IDO transfection on Tregs. (A) Representative FACS scatter plots of the CD4⁺CD25⁺CD127^- T cells in CD3⁺ T cells 7 days after incubation. (B) Representative FACS scatter plots of CD4⁺CD25⁺CD127^- T cells 7 days after co-culture with CHO/EGFP cells. (C) Representative FACS scatter plots of CD₄⁺CD₂₅⁺CD₁₂₇^- T cells 7 days after co-culture with IDO⁺ CHO cells. (D) Representative FACS scatter plots of CD₄⁺CD₂₅⁺CD₁₂₇^- T cells 7 days after co-culture with IDO⁺ CHO cells and inhibitor 1-MT. (P2 region represents CD4⁺ T cells, Q4 region represents CD4⁺CD25⁺CD127^- T cells.) (E) Relative percentages of CD4⁺CD25⁺CD127^- T cells in CD4⁺ T cells. The columns showed the average (%) ± SD from 3 independent experiments. IDO⁺ CHO cells had more Tregs in T cells after co-culture than in control groups. The differences were statistically significant (P < 0.05).