Expression of PKCε in renal cell carcinoma (RCC) cell lines. A. Western blot shows that PKCε is expressed in all five RCC cell lines, with the highest level in 769P cells. GAPDH is the loading control. B. Immunocytochemical staining with PKCε antibody shows that PKCε is mainly expressed in cytoplasm and nuclei of 769P cells (original magnification×200). Green fluorescence indicates PKCε-positive cells, whereas blue fluorescence indicates the nuclei of the cells. The first panel is a merge image of the latter two.