Figure 4

Knockdown of PKCε sensitizes 769P cells to Sunitinib (A) and 5-fluorouracil (B). 769P cells were transfected with PKCε siRNA or control siRNA; untransfected cells were used as blank control. At 72 h after siRNA transfection, cells were treated with sunitinib (0.2, 1, and 5 μM) or 5-fluorouracil (1.25, 2.5, and 5 μg/ml) for another 48 h. MTT assay shows increased sensitivity of cells to sunitinib and 5-fluorouracil after siRNA transfection (**, P < 0.01).