Figure 2

Immunoblot analysis of galectin-3, E-cadherin, and villin in normal kidney, intermediate and tumor tissues as well as RC-124 and RCC-FG1 cells. A, Protein contents in homogenates from tissue samples of 39 patients were measured. Equal protein amounts were separated by SDS-PAGE followed by immunoblot analysis with anti-galectin-3, -E-cadherin or -villin. One representative blot is depicted. B, Quantitative immunoblot analysis of galectin-3, villin and E-cadherin in normal and tumor tissue. C, Relative variation of galectin-3, villin and E-cadherin in CCRCC to the corresponding normal tissue of each patient. D, Immunoblot analysis of galectin-3, E-cadherin and GAPDH in the lysates of epithelial cells from human kidney (RC-124) in comparison to the clear cell renal cell carcinoma cell line (RCC-FG1) E, Quantification of 3 individual experiments.