Figure 1

Transfection was successful and levels of JMJD2A mRNA and protein were both down-regulated. A. The green fluorescence cells transfected with FAM-siRNA under fluorescence microscope (Note: ×100). B. Column diagram analysis for mRNA levels of JMJD2A. JMJD2A-specific siRNA resulted in the reduction of JMJD2A mRNA levels in MDA-MB-231 cells. C. Western blot analysis for JMJD2A protein. D. Column diagram analysis for optical density by Western blotting. JMJD2A protein levels were down-regulated in siRNA group. (*P < 0.05, compared with blank control group and negative control group respectively)