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Figure 2 | Journal of Experimental & Clinical Cancer Research

Figure 2

From: Improved retroviral suicide gene transfer in colon cancer cell lines after cell synchronization with methotrexate

Figure 2

Infection efficiency of the β- gal retroviral vector. DHDK12 cells (A) and HT29 cells (B) were treated for 24 hr with (filled circle) or whithout (open circle) MTX. Cells were transduced with TG 5391 at the indicated times after MTX removal. The level of β-galactosidase activity was obtained 48 hr after the transduction by flow cytometry analysis using FDG, a fluorescent substrate of β-galactosidase. The percentage of cells in S phase (open triangle) at various time after MTX removal was determined by flow cytometry analysis of DNA content. Data are expressed as the mean ± SE from at least three separate experiments.

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